Description
The pEXP series of expression vectors, developed by Aleksei Lulla (from Marko’s lab in Cambridge), are for parallel cloning of fusion constructs (in particular) for screening of soluble and folded forms of your target proteins.
These new vectors are based on the pOP series (and all the old benefits of these and their ancesteros pBAT series), but they are of course improved upon the old, not least in their multiple cloning site which is designed for sequence and ligation independent cloning (SLIC).
When using these vectors, please cite them as Aleksei Lulla et. al, unpublished.
Materials
pExp-NHis Plasmid map Request
Contains mini-cistron (PMID: 3534891), N-terminal 8xHis followed by TEV protease cleavage site, optional C-terminal StrepII-tag. Cloning of GOI between BsaI and XhoI or HindIII.
pExp-CHis Plasmid map Request
Contains mini-cistron (PMID: 3534891), C-terminal 8xHis. Cloning of GOI between NcoI and XhoI or HindIII.
pExp-Bla Plasmid map Request
Contains N-terminal 8xHis, fusion partner: Bla (beta-lactamase from the halophile Chromohalobacter sp. 560, UniProKB: Q76LX5, PMID: 20838790) followed by TEV protease cleavage site, optional C-terminal StrepII-tag. Cloning of GOI between BsaI and XhoI or HindIII.
Fusion MW: 42.2 Da 390 residues pI: 4.3428
pExp-DsbA Plasmid map Request
Contains N-terminal 8xHis, fusion partner: DsbA (leaderless thiol:disulfide interchange protein DsbA from E. coli, UniProtKB: P0AEG4) followed by TEV protease cleavage site, optional C-terminal StrepII-tag. Cloning of GOI between BsaI and XhoI or HindIII.
pExp-DsbC Plasmid map Request
Contains N-terminal 8xHis, fusion partner: DsbC (leaderless thiol:disulfide interchange protein DsbC from E. coli, UniProtKB: P0AEG6, PMID: 23607455) followed by TEV protease cleavage site, optional C-terminal StrepII-tag. Cloning of GOI between BsaI and XhoI or HindIII.
Fusion MW: 26.2 Da 240 residues pI: 6.7324
pExp-GB1 Plasmid map Request
Contains N-terminal 8xHis, fusion partner: GB1 (domain B1 of Immunoglobulin G-binding protein G from Streptococcus sp., UniProtKB: P19909, PMID: 9385638) followed by TEV protease cleavage site, optional C-terminal StrepII-tag. Cloning of GOI between BsaI and XhoI or HindIII.
Fusion MW: 8.8 Da 79 residues pI: 6.2792
pExp-GST Plasmid map Request
Contains N-terminal 8xHis, fusion partner: MBP (Glutathione S-transferase from Schistosoma japonicum, UniProtKB: P08515, PMID: 3047011) followed by TEV protease cleavage site, optional C-terminal StrepII-tag. Cloning of GOI between BsaI and XhoI or HindIII.
Fusion MW: 28.1 Da 241 residues pI: 6.8621
pExp-MBP Plasmid map Request
Contains N-terminal 8xHis, fusion partner: MBP (maltose-binding protein (malE) from E. coli, UniProtKB: P0AEX9, PMID: 10452611) followed by TEV protease cleavage site, optional C-terminal StrepII-tag. Cloning of GOI between BsaI and XhoI or HindIII.
Fusion MW: 43.1 Da 391 residues pI: 5.4478
pExp-sfGFP Plasmid map Request
Contains N-terminal 8xHis, fusion partner: sfGFP (superfolder green fluorescent protein, PMID: 16369541) followed by TEV protease cleavage site, optional C-terminal StrepII-tag. Cloning of GOI between BsaI and XhoI or HindIII.
Fusion MW: 29.5 Da 262 residues pI: 6.6866
pExp-Sumo Plasmid map Request
Contains N-terminal 8xHis, fusion partner: Sumo (Smt3 from Saccharomyces cerevisiae, UniProtKB: Q12306, PMID: 16084395) followed by TEV protease cleavage site, optional C-terminal StrepII-tag. Cloning of GOI between BsaI and XhoI or HindIII.
Fusion MW: 13.8 Da 119 residues pI: 6.1568
pExp-Trx Plasmid map Request
Contains N-terminal 8xHis, fusion partner: Trx (thioredoxin (trxA) from E. coli, UniProtKB: P0AA25, PMID: 7763371) followed by TEV protease cleavage site, optional C-terminal StrepII-tag. Cloning of GOI between BsaI and XhoI or HindIII.