Description
DNA molecular weight markers, also known as DNA ladders, are used to compare the size of DNA fragments run on electrophoresis gels with fragments of known sizes.
We have plasmids for producing both 100 bp and 1 kb DNA ladders, by digestion with common restriction enzymes.
Protocol summary
Digest the plasmids with the restriction enzymes in the protocol, and check the digestion by running agarose gels. Then, dilute them as recommended.
Materials
pPSU1 and pPSU2 plasmids Request
References
Henrici, R.C., Pecen, T.J., Johnston, J.L. et al. The pPSU Plasmids for Generating DNA Molecular Weight Markers. Sci Rep 7, 2438 (2017). https://doi.org/10.1038/s41598-017-02693-1