E. coli strains Resistance Recommended use
DH5α Request None Plasmid cloning and DNA propagation
BL21 (DE3) Request None General protein expression
BL21 (DE3) TrxB Request Kan Proteins containing disulfide bonds
BL21 (DE3) pLysS Request Cm Expression of toxic proteins
BL21 (DE3) AI Request None Tunable protein expression with arabinose
Rosetta2 (DE3) Request Cm Genes enriched in rare codons
Origami (DE3) Request Kam, Tet Proteins with multiple disulfide bonds
Tuner (DE3) Request None Fine control of protein expression
ShuffleT7 express Request Strep, Spec Expression of disulfide-bonded proteins
Lemo21 (DE3) Request Cm Expression of toxic/difficult proteins
XL10 Gold Request Tet, Cm Difficult cloning applications
T7 Express Request None Rapid protein expression
XL1 Blue Request Tet Plasmid cloning and DNA propagation
SoluBL21 (DE3) Request None Soluble protein expression
Nico (DE3) Request None Expression with enhanced purity of IMAC- purified target proteins
BL21 (DE3) BirA Request Cm In vivo biotinylation
C41 (DE3) Request None Expression of toxic and membrane proteins
C43 (DE3) Request None Expression of toxic and membrane proteins
dam-/dcm- Request Strep, Cm Growth of plasmids free of Dam and Dcm methylation
TOP10F Request Strep, Tet Cloning and plasmid propagation
Cm: Chloramphenicol
Kan: Kanamycin
Tet: Tetracyclin
Strep: Streptomycin
Spec: Spectinomycin