Description
We have a construct for production of a high-fidelity DNA polymerase. It is codon-optimised for expression in E.coli.
Protocol summary
The plasmid is transformed to an E. coli strain for T7 expression system, typically BL21(DE3), for protein expression.
Purification is performed using Ni-NTA agarose and Heparin chromatography.
Materials
pRSF_HFpolymerase plasmid Plasmid map Request
Purified HF polymerase Request Datasheet for Phusion DNA polymerase